The PDF file you selected should load here if your Web browser has a PDF reader plug-in installed (for example, a recent version of Adobe Acrobat Reader).
If you would like more information about how to print, save, and work with PDFs, Highwire Press provides a helpful Frequently Asked Questions about PDFs.
Alternatively, you can download the PDF file directly to your computer, from where it can be opened using a PDF reader. To download the PDF, click the Download link above.
How to cite (IEEE):
-. Mardalisa, S. Suhandono, N. Yanti, F. Rozi, F. Nova, & -. Primawati
"Bioinformatic Analysis in Designing Mega-primer in Overlap Extension PCR Cloning (OEPC) Technique," JOIV : International Journal on Informatics Visualization, vol. 5, no. 2, , pp. 139-143, May. 2021.
https://doi.org/10.30630/joiv.5.2.459
How to cite (APA):
Mardalisa, -., Suhandono, S., Yanti, N., Rozi, F., Nova, F., & Primawati, -.
(2021).
Bioinformatic Analysis in Designing Mega-primer in Overlap Extension PCR Cloning (OEPC) Technique.
JOIV : International Journal on Informatics Visualization, 5(2), 139-143.
https://doi.org/10.30630/joiv.5.2.459
How to cite (Chicago):
Mardalisa, -, Suhandono, Sony, Yanti, Novi, Rozi, Fazrol, Nova, Fitri, AND Primawati, -.
"Bioinformatic Analysis in Designing Mega-primer in Overlap Extension PCR Cloning (OEPC) Technique" JOIV : International Journal on Informatics Visualization [Online], Volume 5 Number 2 (7 May 2021)
https://doi.org/10.30630/joiv.5.2.459
How to cite (Vancouver):
Mardalisa -, Suhandono S, Yanti N, Rozi F, Nova F, & Primawati - .
Bioinformatic Analysis in Designing Mega-primer in Overlap Extension PCR Cloning (OEPC) Technique.
JOIV : International Journal on Informatics Visualization [Online]. 2021 May;
5(2):139-143.
https://doi.org/10.30630/joiv.5.2.459
How to cite (Harvard):
Mardalisa, -., Suhandono, S., Yanti, N., Rozi, F., Nova, F., & Primawati, -.
,2021.
Bioinformatic Analysis in Designing Mega-primer in Overlap Extension PCR Cloning (OEPC) Technique.
JOIV : International Journal on Informatics Visualization, [Online] 5(2), pp. 139-143.
https://doi.org/10.30630/joiv.5.2.459
How to cite (MLA8):
Mardalisa, -, Sony Suhandono, Novi Yanti, Fazrol Rozi, Fitri Nova, & - Primawati.
"Bioinformatic Analysis in Designing Mega-primer in Overlap Extension PCR Cloning (OEPC) Technique." JOIV : International Journal on Informatics Visualization [Online], 5.2 (2021): 139-143. Web. 25 Sep. 2023
, https://doi.org/10.30630/joiv.5.2.459
BibTex Citation Data :
@article{JOIV459,
author = {- Mardalisa and Sony Suhandono and Novi Yanti and Fazrol Rozi and Fitri Nova and - Primawati},
title = {Bioinformatic Analysis in Designing Mega-primer in Overlap Extension PCR Cloning (OEPC) Technique},
journal = {JOIV : International Journal on Informatics Visualization},
volume = {5},
number = {2},
year = {2021},
keywords = {Bioinformatics; mega-primer design; Overlap Extension PCR Cloning (OEPC); genetic algorithm.},
abstract = {Bioinformatics has developed into an application tool for basic and applied research in the biomedical and biotechnology field. Polymerase Chain Reaction (PCR) is a common technique in the molecular area that has always involved bioinformatics science. PCR cloning techniques such as TA cloning and PCR-mediated cloning exhibit complex processes with low success rates. One easy, effective, and practical solution is to use a mega-primer with the Overlap Extension PCR Cloning (OEPC) technique. The success of PCR cloning using the mega-primer design in the OEPC technique is strongly influenced by the characteristics of the mega-primer used. Knowledge of mega-primer characteristics is one of the important factors in the success of PCR cloning. The design process for the mega-primer str promoter was characterized based on the principle of a genetic algorithm using the web-based bioinformatics tools such as ClustalW, NetPrimer, and BLAST. The success of the mega-primer construction in producing recombinant pSB1C3 vector has been confirmed by the sequencing method and the function of the reporting protein (AmilCP). DNA analysis shows a 100 % homologous sequence on the str promoter, while E. coli colonies successfully express the purplish-blue color. Mega-primer characters can save costs and time of the research by maintaining the primer parameters that provide optimal values and increase the success value of PCR cloning via bioinformatics software. Hence, implications on biological problems, especially using DNA and amino acid sequences, could solve rapidly.},
issn = {2549-9904}, pages = {139--143}, doi = {10.30630/joiv.5.2.459},
url = {https://joiv.org/index.php/joiv/article/view/459}
}
Refworks Citation Data :
@article{{JOIV}{459}, author = {Mardalisa, -., Suhandono, S., Yanti, N., Rozi, F., Nova, F., Primawati, -.}, title = {Bioinformatic Analysis in Designing Mega-primer in Overlap Extension PCR Cloning (OEPC) Technique}, journal = {JOIV : International Journal on Informatics Visualization}, volume = {5}, number = {2}, year = {2021}, doi = {10.30630/joiv.5.2.459}, url = {} }Refbacks
- There are currently no refbacks.
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
__________________________________________________________________________
JOIV : International Journal on Informatics Visualization
ISSN 2549-9610 (print) | 2549-9904 (online)
Organized by Department of Information Technology - Politeknik Negeri Padang, and Institute of Visual Informatics - UKM and Soft Computing and Data Mining Centre - UTHM
W : http://joiv.org
E : joiv@pnp.ac.id, hidra@pnp.ac.id, rahmat@pnp.ac.id
View JOIV Stats
is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.